Histone H1 (Nuclear Marker) (clone SPM256), anti-human, mouse, rat
€0.00
In stock
SKU
ARP-24-3005-MSM1X
Catalog Number: 24-3005-MSM1X
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG2a, kappa
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG2a, kappa
Decription:
Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Synonyms: H1(0), H1.1, H1.2, H1.3, H1.4, H1.5, H10, H1A, H1F0, H1F1, H1F2, H1F3, H1F4, H1F5, H1FNT, H1FOO, H1FT, H1FV, H1FX, H1t, H1T2, H1X, HANP1, His1, HisC, HIST1, HIST1H1A, HIST1H1B, HIST1H1C, HIST1H1D, HIST1H1E, HIST1H1T, Oocyte-specific histone H1, Testicular H1 histone
Clone: SPM256
Isotype: Mouse IgG2a, kappa
Immunogen: Nuclei of human leukemia biopsy cells
Reactivity: human, mouse and rat. Others-not known
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Reactivity: HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma.
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Synonyms: H1(0), H1.1, H1.2, H1.3, H1.4, H1.5, H10, H1A, H1F0, H1F1, H1F2, H1F3, H1F4, H1F5, H1FNT, H1FOO, H1FT, H1FV, H1FX, H1t, H1T2, H1X, HANP1, His1, HisC, HIST1, HIST1H1A, HIST1H1B, HIST1H1C, HIST1H1D, HIST1H1E, HIST1H1T, Oocyte-specific histone H1, Testicular H1 histone
Clone: SPM256
Isotype: Mouse IgG2a, kappa
Immunogen: Nuclei of human leukemia biopsy cells
Reactivity: human, mouse and rat. Others-not known
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Reactivity: HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma.
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
| Is Featured? | No |
|---|
Write Your Own Review