Histone H1 Polyclonal Antibody

Background:
May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.

Research Area: Cancer, Epigenetics and Nuclear Signaling

Synonyms:
HIST1H1B, H1F5, Histone H1.5, Histone H1a, Histone H1b, Histone H1s-3, HIST1H1D, H1F3, Histone H1.3, Histone H1c, Histone H1s-2, HIST1H1E, H1F4, Histone H1.4, Histone H1b, Histone H1s-4

Immunogen: Synthesized peptide derived from human Histone H1 around the non-phosphorylation site of Thr17.

Swissprot: P16401,P16402,P10412

Calculated MW: 23 kDa
Observed MW: 31, 28 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Concentration: 1 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:20000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.