Histone H2A.X (Phospho-Ser139) polyclonal, anti-human, mouse, rat

Specificity:
Histone H2A.X (Phospho-Ser139) Antibody detects endogenous levels of H2AFX only when phosphorylated at Ser139.

Synonyms: H2A.X, H2AFX, H2a/x, HIST5-2AX, Histone H2A.X

Immunogen:
The antiserum was produced against synthesized phospho-peptide derived from human Histone H2A.X around the phosphorylation site of Ser139.

Immunogen Sequence:
Phospho

Modification Sites: H:S139, M:S139, R:S140

MW (kDa): 15 kDa

NCBI Gene Symbol: H2AFX

Database Links:
Gene ID: 3014
Swiss Prot No.: P16104
OMIM No.: 601772
Unigene No.: Hs.477879

Application Dilutions: WB: 1:500~1:1000, ELISA: 1:10000

Purification:
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Physical Format:
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Concentration: 1 mg/ml

Storage: Stable at -20°C for at least 1 year.