Histone H2A.X Polyclonal Antibody

Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Research Area: Cancer, Epigenetics and Nuclear Signaling

Synonyms:
H2A histone family member X, H2A histone family member X, H2A.FX, H2A.X, H2a/x, H2AFX, H2AX, H2AX, Histone H2A.X

Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse Histone H2A.X

Swissprot: P16104,P27661

Calculated MW: 15-18 kDa
Observed MW: 18 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4

Concentration: 0.86 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:300-1:800

Storage:
Store at -20°C. Avoid freeze / thaw cycles.