Histone H2B Monoclonal Antibody

Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. HIST1H2BB (Histone Cluster 1 H2B Family Member B) is a Protein Coding gene. Among its related pathways are DNA Double-Strand Break Repair and Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3. GO annotations related to this gene include sequence-specific DNA binding and protein heterodimerization activity. An important paralog of this gene is HIST1H2BN.

Research Area: Cancer, Epigenetics and Nuclear Signaling

Synonyms:
HIST1H2BA, testis, bA317E16.3, H2B, H2B histone family member U, H2B histone family member U testis specific, H2B histone family, member U, (testis specific), H2B testis, H2B1A, H2BFU, H2BT, HIST1H2BA, Histone 1, H2ba, Histone cluster 1 H2ba, Histone H2B, Histone H2B testis, Histone H2B type 1 A, Histone H2B type 1-A, Histone H2B type 1A, STBP, Testis specific histone H2B, Testis-specific histone H2B, TSH 2B, TSH2B, TSH2B.1

Immunogen: Synthetic Peptide

Swissprot: P33778

Calculated MW: 14 kDa
Observed MW: 14 kDa

Purification Method: Protein A purification

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Concentration: 1 mg/mL
Dilution: WB 1:1000-3000, IF 1:100-200

Storage:
Store at -20°C. Avoid freeze / thaw cycles.