Histone H3.3 Polyclonal Antibody

Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.

Research Area: Cancer, Epigenetics and Nuclear Signaling

Synonyms:
H3F3A, H3.3A, H3F3, histone H3.3

Immunogen: Recombinant fusion protein of human H3F3A (NP_002098.1).

Swissprot: P84243
Gene ID: 3020

Calculated MW: 15 kDa
Observed MW: 16 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:2000"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.