Histone H4 (tri methyl K20) polyclonal, anti-human, mouse, rat

Background:
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fibers. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form the octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Human and mouse Histone H4 are subject to trimethylation at Lys 20, a modification that may be necessary for select DNA transactions or chromatin state transitions.

Alternative Name:
HIST1H4A, Histone gene cluster 1, H4A, Histone gene cluster 2, H4, dJ160A22.1, dJ160A22.2, dJ221C16.1, dJ221C16.9, FO108, H4, H4 histone family, member A, H4 histone family, member B, H4 histone family, member C, H4 histone family, member D, H4 histone family, member E, H4 histone family, member G, H4 histone family, member H, H4 histone family, member I, H4 histone family, member J, H4 histone family, member K, H4 histone family, member M, H4 histone family, member N, H4 histone, family 2, H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4/p, H4, H4F2, H4F2iii, H4F2iv, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, HIST1 cluster, H4A, HIST1 cluster, H4B, HIST1 cluster, H4D, HIST2H4, Hist4 cluster, H4, Hist4h4, histone 1, H4a, histone 1, H4c, histone 1, H4d, histone 1, H4f, histone 1, H4h, histone 1, H4i, histone 1, H4j, histone 1, H4k, histone 1, H4l, histone 2, H4a, histone 2, H4b, Histone 4 family, member M, histone 4, H4, histone cluster 1, H4, histone cluster 1, H4a, histone cluster 1, H4b, histone cluster 1, H4c, histone cluster 1, H4d, histone cluster 1, H4e, histone cluster 1, H4f, histone cluster 1, H4h, histone cluster 1, H4i, histone cluster 1, H4j, histone cluster 1, H4k, histone cluster 1, H4l, histone cluster 2, H4a, histone cluster 2, H4b, histone cluster 4, H4, Histone family, member A, Histone family, member B, Histone family, member D, Histone family, member H, Histone family, member I, Histone family, member L, Histone gene cluster 1, H4, Histone gene cluster 1, H4D, Histone gene cluster 1, H4E, Histone gene cluster 1, H4K, Histone gene cluster 4, H4, Histone gene cluster 4, H4 histone, Histone H4, histone IV, family 2,

Application Dilution:
WB:1:1,000
IHC:1:50-1:200

Specificity: Histone H4 (tri methyl K20) polyclonal antibody detects endogenous levels of Histone H4 protein only when tri-methylated at K20.

Immunogen:
recombinant protein

MW: 11 kDa

Swiss-Prot: P62805(Human) P62806(Mouse)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.