HLA-DRB4 Polyclonal Antibody

Background:
HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB4 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.

Research Area: Immunology

Synonyms:
DR 4, DR beta 4 chain, DR4, DRB1 transplantation antigen , DRB4, DRB4, HLA class II histocompatibility antigen, HLA class II histocompatibility antigen DR beta 4 chain , HLA-DRB4, Human leucocyte antigen DRB4 , Leukocyte antigen , Major histocompatibility complex class II DR beta 4 , MHC class II antigen DRB4, MHC class II antigen HLA DR beta , MHC class2 antigen , MHC HLA DR-beta chain

Immunogen: Fusion protein of human HLA-DRB4

Swissprot: P13762
Gene Accession: BC005312

Calculated MW: 30 kDa
Observed MW: Refer to figures

Purification Method: Antigen affinity purification

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Concentration: 0.84 mg/mL
Dilution: WB 1:500-1:2000, ELISA 1:5000-1:10000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.