HMGB1 polyclonal, anti-human,mouse,rat

Background:
Like the histones, HMGB1, also known as high-mobility group protein 1 (HMG-1) is among the most important chromatin proteins. In the nucleus HMGB1 interacts with nucleosomes, transcription factors, and histones. This nuclear protein organizes the DNA and regulates transcription. After binding, HMGB1 bends DNA, which facilitates the binding of other proteins. HMGB1 is secreted by immune cells through leaderless secretory pathway. Activated macrophages and monocytes secrete HMGB1 as a cytokine mediator of Inflammation. In recent research, HMGB1 has been reported as a novel biomarker for human ovarian cancer.

Alternative Name:
HMG-1, Amphoterin, Chromosomal protein, nonhistone, HMG1, DKFZp686A04236, High mobility group 1, High mobility group box 1, High mobility group protein 1, High mobility group protein B1, high-mobility group (nonhistone chromosomal) protein 1, HMG-1, HMG1, HMG3, HMGB 1, HMGB1, HMGB1, NONHISTONE CHROMOSOMAL PROTEIN HMG1, SBP 1, Sulfoglucuronyl carbohydrate binding protein,

Application Dilution:
WB:1:500-1:1,000
ICC:1:200
IHC:1:200
FC:1:50-1:100

Specificity: HMGB1 polyclonal antibody detects endogenous levels of HMGB1 protein.

Immunogen:
peptide

MW: 25 kDa

Swiss-Prot: P09429

Purification & Purity:
Peptide affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.