HNF-4-alpha polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Hepatocyte nuclear factor 4 alpha (HNF4A), also known as NR2A1, is a nuclear receptor that in humans is encoded by the HNF4A gene. It is mapped to 20q13.12. HNF4A is a nuclear transcription factor that binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene plays a role in development of the liver, kidney, and intestines. HNF4A is required for the PXR and CAR-mediated transcriptional activation of CYP3A4. This gene also plays a pivotal role in the expression and synthesis of SHBG, an important glycoprotein made primarily in the liver, which in addition to lowering insulin-resistance also serves in reducing levels of free Oestrogen as-well as prolonging the half-life of Testosterone.

Description:
Rabbit IgG polyclonal antibody for Hepatocyte nuclear factor 4-alpha (HNF4A) detection. Tested with WB, IHC-P, IHC-F in Human, Mouse, Rat.

Synonyms:
FLJ39654 antibody, Hepatic nuclear factor 4 alpha antibody, Hepatocyte nuclear factor 4 alpha antibody, Hepatocyte nuclear factor 4 antibody, Hepatocyte nuclear factor 4-alpha antibody, HNF 4 alpha antibody, HNF 4 antibody, HNF-4-alpha antibody, HNF4 antibody, HNF4 antibody, HNF4A antibody, HNF4A_HUMAN antibody, HNF4a7 antibody, HNF4a8 antibody, HNF4a9 antibody, Hnf4alpha antibody, HNF4alpha10/11/12 antibody, MODY 1 antibody, MODY antibody, MODY1 antibody, NR2A1 antibody, NR2A1 antibody, NR2A21 antibody, Nuclear receptor subfamily 2 group A member 1 antibody, OTTHUMP00000031060 antibody, OTTHUMP00000031062 antibody, TCF 14 antibody, TCF antibody, TCF-14 antibody, TCF14 antibody, TCF14 antibody, Tcf4 antibody, Transcription factor 14, hepatic nuclear factor antibody, Transcription factor 14 antibody, Transcription factor HNF 4 antibody, Transcription factor HNF-4 antibody, Transcription factor HNF4 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
E.coli-derived human HNF-4-alpha recombinant protein (Position: Q164-I474). Human HNF-4-alpha shares 95% and 96% amino acid (aa) sequences identity with mouse and rat HNF-4-alpha, respectively.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P, IHC-F

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.