Horse aHSG (Alpha-2-Heremans Schmid Glycoprotein) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Heremans Schmid Glycoprotein(aHSG). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-2-Heremans Schmid Glycoprotein(aHSG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Heremans Schmid Glycoprotein(aHSG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-2-Heremans Schmid Glycoprotein(aHSG) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Infection immunity

Alternative Names:
HSGa, A2HS, AHS, FETUA, HSG-A2, Fetuin-A, Alpha 2-HS Glycoprotein, Fetuin A, Alpha-2-Z-Globulin, Ba-alpha-2-Glycoprotein

Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 400 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only