Horse Albumin polyclonal, anti-equine

Quantity: 200 µl

Background:
The defined antibody specificity is directed to albumin as tested against horse sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against horse serum and the purified albumin. As precipitating antiserum to identify or measure horse albumin by a variety of immunodiffusion techniques, including immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.

Isotype: Rabbit Polyclonal

Source: Highly purified albumin isolated from horse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Reactivity: Equine

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiserum has been tested for cross-reactivity in double radial immunodiffusion with the following results: Bovine + Cat + Chicken - Dog + Duck - Goat + Guinea Pig + Hamster ++ Human + Monkey ++ Mouse + pigeon - Rabbit ﾱ Rat + Sheep + Swine + A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.