Horse DBP (Vitamin D Binding Protein) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin D Binding Protein(DBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vitamin D Binding Protein(DBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vitamin D Binding Protein(DBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin D Binding Protein(DBP) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Metabolic pathway, Tumor immunity,

Alternative Names:
GC, VDBG, VDBP, Group-Specific Component, Gc-globulin

Sample Type:
serum, plasma and other biological fluids

Assay Type: Sandwich

Standard: 400 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only