Horse IL1RA (Interleukin 1 Receptor Antagonist) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Antagonist(IL1RA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Antagonist(IL1RA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Antagonist(IL1RA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Antagonist(IL1RA) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cytokine, Signal transduction, Infection immunity

Alternative Names:
IL1RN, IL1-RA, ICIL1RA, IL1ra3, IL1F3, IRAP, IL-1RA, Interleukin-1 Family Member 3, IL1 inhibitor, Anakinra

Sample Type:
Serum, plasma and other biological fluids.

Assay Type: Sandwich

Standard: 5000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only