HRAS 293 Cell Lysate
€447.00
In stock
SKU
401480
Protein Family: GTP-binding Proteins, Transporters
Pathway & Disease: Signal Transduction, Cell Motility, Cell Communication, Immune System, Endocrine System, Nervous and Sensory Systems, Development and Behavior, Cancers
Description:
Antigen standard for v-Ha-ras Harvey rat sarcoma viral oncogene homolog (HRAS), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC216409, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Alternate Names: c-has/bas p21 protein, c-ras-Ki-2 activated oncogene, transformation gene: oncogene HAMSV, Ha-Ras1 proto-oncoprotein, Ras family small GTP binding protein H-Ras, p19 H-RasIDX protein, transforming protein p2, HRAS, C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, K-RAS, N-RAS, RASH1
ORF clone URL:
Format: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 100 mM DTT).
Applications: ELISA, WB, IP
Accession No: NP_005334
Application Notes: WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Storages: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Pathway & Disease: Signal Transduction, Cell Motility, Cell Communication, Immune System, Endocrine System, Nervous and Sensory Systems, Development and Behavior, Cancers
Description:
Antigen standard for v-Ha-ras Harvey rat sarcoma viral oncogene homolog (HRAS), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC216409, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Alternate Names: c-has/bas p21 protein, c-ras-Ki-2 activated oncogene, transformation gene: oncogene HAMSV, Ha-Ras1 proto-oncoprotein, Ras family small GTP binding protein H-Ras, p19 H-RasIDX protein, transforming protein p2, HRAS, C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, K-RAS, N-RAS, RASH1
ORF clone URL:
Format: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 100 mM DTT).
Applications: ELISA, WB, IP
Accession No: NP_005334
Application Notes: WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Storages: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
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