HRH3 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
The histamine receptor H3(HRH3) is a presynaptic autoreceptor on histamine neurons in the brain and a presynaptic heteroreceptor in nonhistamine-containing neurons in both the central and peripheral nervous systems. The deduced 445-amino acid HRH3 protein contains 7 predicted transmembrane domains. And it shares 22% and 21.4% amino acid sequence homology with the H1(HRH1) and H2(HRH2) receptors, respectively. The expression of recombinant HRH3 in a variety of cell lines conferred an ability to inhibit adenylate cyclase in response to histamine, but not to acetylcholine or any other biogenic amine. Additionally, HRH3 was most notably observed throughout the thalamus, the ventromedial hypothalamus, and the caudate nucleus. Strong expression was also seen in layers II, V, and VIb of the cerebral cortex, in the pyramidal layers of the hippocampus, and in olfactory tubercle.

Description:
Rabbit IgG polyclonal antibody for Histamine H3 receptor (HRH3) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
G protein coupled receptor 97 antibody, G-protein coupled receptor 97 antibody, GPCR 97 antibody, GPCR97 antibody, H3R antibody, HH 3R antibody, HH3 R antibody, HH3R antibody, Histamine H3 receptor antibody, Histamine receptor H3 antibody, HRH 3 antibody, HRH3 antibody, HRH3_HUMAN antibody, OTTHUMP00000031459 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human HRH3(428-445aa CPQKLKIQPHSSLEHCWK), different from the related rat sequence by three amino acids.A synthetic peptide corresponding to a sequence at the C-terminus of human HRH3(428-445aa CPQKLKIQPHSSLEHCWK), different from the related rat sequence by three amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.