HRI rabbit polyclonal, anti-human, mouse, monkey

Backgroud: The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008],catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Hemin inactivates EIF2AK1 by promoting the formation of a disulfide-linked homodimer. Binding of nitric oxide (NO) to the heme iron in the N-terminal heme-binding domain activates the kinase activity, while binding of carbon monoxide (CO) suppresses kinase activity.,function:Mediates down-regulation of protein synthesis in response to various stress conditions by the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51'. Protein synthesis is inhibited at the level of initiation.,miscellaneous:Can bind 2 molecules of heme per polypeptide chain.,PTM:Activated by autophosphorylation; phosphorylated predominantly on serine and threonine residues, but also on tyrosine residues.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 HRM (heme regulatory motif) repeats.,subunit:Synthesized in an inactive form that binds to the N-terminal domain of CDC37. Has to be associated with a multiprotein complex containing Hsp90, CDC37 and PPP5C for maturation and activation by autophosphorylation. The phosphatase PPP5C modulates this activation. Homodimer; non-covalently bound in the absence of hemin. Converted to an inactive disulfide linked homodimer in the presence of hemin.,tissue specificity:Detected in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, kidney, spleen, muscle and stomach.,

Synonyms: EIF2AK1, HRI, KIAA1369, Eukaryotic translation initiation factor 2-alpha kinase 1, Heme-controlled repressor, HCR, Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase, Heme-regulated inhibitor, Hemin-sensitive initiation factor 2

Gene Name: EIF2AK1

Gene ID: 27102

SwissProt ID: Q9BQI3

Purification: Affinity purification

Storage: Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.