HSF1 (Phospho-S326) polyclonal, anti-human

Background:
Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors 1 and 2 (HSF1 and HSF2), also designated HSTF1 and HSTF2, are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer, whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.

Alternative Name:
HSF 1, HSTF 1, HSF1, Heat shock factor 1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1, hsf1, HSF1, HSTF 1, HSTF1,

Application Dilution:
WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Specificity: HSF1 (Phospho-S326) polyclonal antibody detects endogenous levels of HSF1 protein only when phosphorylated at S326.

Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Ser326 of human HSF1.

MW: 82 kDa

Swiss-Prot: Q00613(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.