HSF1 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
HSF1(HEAT-SHOCK TRANSCRIPTION FACTOR 1), also called HEAT-SHOCK FACTOR 1, is a protein that in humans is encoded by the HSF1 gene. The product of HSF1 gene is a heat-shock transcription factor. The International Radiation Hybrid Mapping Consortium maps the HSF1 gene to chromosome 8. HSF1 exists as an inactive monomer in a complex with Hsp40/Hsp70 and Hsp90. Upon stress, such as elevated temperature, HSF1 is released from the chaperone complex and trimerizes. HSF1 is then transported into the nucleus where it is hyperphosphorylated and binds to DNA containing heat shock elements. HSF1's target genes include major inducible heat shock proteins such as Hsp72, and interestingly, noncoding RNA within Satellite III repeat regions. In a novel in vitro system human HSF1 can be activated by nonnative protein, heat, and geldanamycin. HSR1 is constitutively expressed in human and rodent cells and its homologs are functionally interchangeable. Furthermore, Hsf1-deficient mice had a longer free-running period than wildtype littermates.

Description:
Rabbit IgG polyclonal antibody for Heat shock factor protein 1 (HSF1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.

Synonyms:
Heat shock factor 1 antibody, Heat shock factor protein 1 antibody, Heat shock transcription factor 1 antibody, HSF 1 antibody, hsf1 antibody, HSF1_HUMAN antibody, HSTF 1 antibody, HSTF1 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human HSF1(380-397aa DKNELSDHLDAMDSNLDN), identical to the related rat and mouse sequences.A synthetic peptide corresponding to a sequence in the middle region of human HSF1(380-397aa DKNELSDHLDAMDSNLDN), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB, IHC-P, IHC-F, ICC

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.