HSP27 (clone EBS-O-060), anti-human, mouse, rat, chicken, chimpanzee, monkey, sheep

Source:
A BALB/c mouse was immunized with HSP27 derived from MCF-7.
Fusion partner: NS-1.

Specifications:
EBS-O-060 reacts with heat shock protein 27 (HSP27) in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore HSP27 may be useful in classifying various hormone sensitive tumors.

Species reactivity:
Positive: chicken, chimpanzee, human, monkey, mouse, sheep, rat.

Applications:
Demonstrate HSP27 in IHC, FC, IF, ELISA or Immunoblot.
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate
Western blot: +

Positive control: ~50% Of breast cancers are positive for hsp27 especially those that are also positive for ER and/or PgR.

References:
- Adams, DJ. et al. Cancer Res.43: 4297-4301 (1983).
- Adams, D.J. et al. Cancer Res.45: 2445-2449 (1985).
- Moretti-Rojas, I, et al. Breats Cancer Res. And Treat.11: 155-163 (1988).
- McGuire, S.E. et al. Somatic Cell and Molecular Genetics 15: 167-163 (1988).
- Edwards, D.P et al. Breast Cancer Res.and Treat.1: 209-223 (1981).