HSP60 (clone HSP60-1), anti-human, mouse, rat, bovine, chicken, dog, hamster, monkey, pig, rabbit, sheep

Source:
A BALB/c mouse was immunized with recombinant human HSPD1.
Fusion partner: NS-1.

Specifications:
HSP60-1 reacts with Heat Shock Protein 60 or HSPD1, found in mitochondria. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (HSP’s), more appropriately called as Stress Response Proteins (SRP’s). HSP60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial HSP60, but also against its mammalian homolog.

Species reactivity:
Positive: chicken, cow, dog, hamster, human, monkey, mouse, pig, rabbit, r at, sheep.

Applications:
Demonstrate HSP60 in IHC, FC, IF or Immunoblot.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate
Western blot: +

Positive control: HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Carcinomas.

References:
- Schlesinger, M.J., et al. Heat Shock: from Bacteria to Man. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory (1982)