HSP60 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.

Description:
Rabbit IgG polyclonal antibody for 60 kDa heat shock protein, mitochondrial (HSPD1) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.

Synonyms:
60 kDa chaperonin antibody, 60 kDa heat shock protein mitochondrial antibody, 60 kDa heat shock protein, mitochondrial antibody, CH60_HUMAN antibody, Chaperonin 60 antibody, Chaperonin antibody, Chaperonin, 60-KD antibody, CPN 60 antibody, CPN60 antibody, fa04a05 antibody, GROEL antibody, GroEL Homolog antibody, Heat shock 60kD protein 1 chaperonin antibody, Heat shock 60kDa protein 1 antibody, Heat shock protein 1(chaperonin) antibody, Heat Shock Protein 60 antibody, Heat shock protein 65 antibody, HLD4 antibody, Hsp 60 antibody, HSP 65 antibody, HSP-60 antibody, HSP60 antibody, HSP65 antibody, HSPD 1 antibody, HSPD1 antibody, HuCHA60 antibody, Mitochondrial matrix protein P1 antibody, P60 lymphocyte protein antibody, Short heat shock protein 60 Hsp60s1 antibody, Spastic paraplegia 13 antibody, SPG 13 antibody, SPG13 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of rat HSP60(503-516aa YDAMLGDFVNMVEK), different from the related human sequence by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P, ICC

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.