Hsp60 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.

Description:
Rabbit IgG polyclonal antibody for 60 kDa heat shock protein, mitochondrial (HSPD1) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.

Synonyms:
60 kDa chaperonin antibody, 60 kDa heat shock protein, mitochondrial antibody, 60kDa antibody, cb863 antibody, CH60_HUMAN antibody, Chaperonin 60 antibody, Chaperonin antibody, Chaperonin, 60-KD antibody, CPN60 antibody, GROEL antibody, GroEL, E. coli, homolog of antibody, Heat shock 60kD protein 1 (chaperonin) antibody, heat shock 60kDa protein 1 (chaperonin) antibody, Heat shock 60kDa protein 1 antibody, Heat shock protein 1 (chaperonin) antibody, Heat shock protein 60 antibody, Heat shock protein 65 antibody, HLD4 antibody, Hsp 60 antibody, HSP 65 antibody, HSP-60 antibody, HSP60 antibody, HSP65 antibody, HSPD1 antibody, HuCHA60 antibody, Mitochondrial matrix protein P1 antibody, P60 lymphocyte protein antibody, Short heat shock protein 60 Hsp60s1 antibody, Spastic paraplegia 13 (autosomal dominant) antibody, SPG13 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
E.coli-derived human Hsp60 recombinant protein (Position: A260-Q496). Human Hsp60 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp60.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P, ICC

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.