HSP70 (clone BRM-22), anti-human, rat, bovine, rabbit

Background:
Heat-shock proteins, or stress proteins, are expressed in response to heat shock and a variety of other stress stimuli including oxidative free radicals and toxic metal ions. Sargent et al. identified a duplicated HSP70 locus in the class III region of the major histocompatibility complex on 6p21.3. A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex(MHC) class III region 92 kilobases(kb) telomeric to the C2 gene. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.

Decription:
Mouse IgG monoclonal antibody for HSP70, heat shock 70kDa protein 1A, heat shock 70kDa protein 1B (HSPA1A, HSPA1B) detection. Tested with WB, IHC-P, IHC-F in Human, bovine, rabbit, rat. No cross reactivity with other proteins.

Synonyms: HSPA1, Hsp70-1, Hsp70-2, Heat shock 70 kDa protein 1/2

Clone: BRM-22

Isotype: Mouse IgG1

Immunogen: HSP70 isolated from bovine brain.

Reactivity: human, bovine, rabbit, rat

Form: Lyophilized; Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Applications: WB, IHC-P, IHC-F

Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.