HSP70 polyclonal, anti-human, mouse, rat

Background:
The 70 kilodalton heat shock proteins (Hsp70s) are a family of conserved ubiquitously expressedheat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. When not interacting with a substrate peptide, Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity, such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the?ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues, and interacts with them. This spontaneous interaction is reversible, and in the ATP bound state Hsp70 may relatively freely bind and release peptides. However, the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis.

Alternative Name:
HSP70-1, HSP70.1, DnaK type molecular chaperone HSP70 1, Epididymis secretory protein Li 103, FLJ54303, FLJ54370, FLJ54392, FLJ54408, FLJ75127, Heat shock 70 kDa protein 1, Heat shock 70 kDa protein 1/2, Heat shock 70 kDa protein 1A/1B, Heat shock 70kDa protein 1A, Heat shock 70kDa protein 1B, Heat shock induced protein, HEL S 103, HSP70 1, HSP70 1B, HSP70 2, HSP70-1/HSP70-2, HSP70-1A, HSP70.1, HSP70.1/HSP70.2, HSP70I, HSP71, HSP72, HSPA1, HSPA1A, HSPA1B,

Application Dilution:
WB:1:500
ICC:1:200
IHC:1:200

Specificity: HSP70 polyclonal antibody detects endogenous levels of HSP70 protein.

Immunogen:
peptide

MW: 70 kDa

Swiss-Prot: P0DMV8

Purification & Purity:
Peptide affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.