Human 8-iso prostaglandin F2α (8-iso-PGF2a) ELISA kit
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In stock
SKU
ELK8402
Catalog Number: ELK8402
Reactivity: Human
Standard Range: 31.25-2000 pg/mL
Sensitivity: 8.2 pg/mL
Information
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Reactivity: Human
Standard Range: 31.25-2000 pg/mL
Sensitivity: 8.2 pg/mL
Information
Request Manual
Questions? Contact us!
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-iso-PGF2α(8-isoprostane) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 8-iso-PGF2α(8-isoprostane). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-iso-PGF2α(8-isoprostane) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Endocrinology, Pulmonology, Hepatology, Hormone metabolism
Alternative Names:
8-isoprostane, 8-iso-PGF2α
Sample Type:
serum, plasma and other biological fluids
Assay Type: Competitive Inhibition
Standard: 2000 pg/mL
Assay Length: 2h
Assay Length: For Research Use Only
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-iso-PGF2α(8-isoprostane) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 8-iso-PGF2α(8-isoprostane). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-iso-PGF2α(8-isoprostane) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Endocrinology, Pulmonology, Hepatology, Hormone metabolism
Alternative Names:
8-isoprostane, 8-iso-PGF2α
Sample Type:
serum, plasma and other biological fluids
Assay Type: Competitive Inhibition
Standard: 2000 pg/mL
Assay Length: 2h
Assay Length: For Research Use Only
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