Human ACA-IgG (Anti-Cardiolipin Antibody IgG) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with Cardiolipin IgG protein. Standards or samples are added to the appropriate microtiter plate wells that with Cardiolipin IgG protein. Then add a biotin-conjugated Cardiolipin IgG protein. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anti-Cardiolipin Antibody IgG(ACA-IgG) , biotin-conjugated protein and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Cardiolipin Antibody IgG(ACA-IgG) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cardiovascular biology

Alternative Names:
ACA-IgG, Anti-Cardiolipin Antibody IgG

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids

Assay Type: Sandwich

Standard: 200 ng/mL

Assay Length: 3.5h

Assay Length: For Research Use Only