Human ACP5/TRACP ELISA Kit
€0.00
In stock
SKU
BEK1368
Catalog Number: BEK1368
Size(s): 96 tests/kit
Standard Range: 78 pg/ml - 5000 pg/ml
Sensitivity: <20 pg/ml
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Size(s): 96 tests/kit
Standard Range: 78 pg/ml - 5000 pg/ml
Sensitivity: <20 pg/ml
Request Information
Introduction:
Tartrate-resistant acid phosphatase (TRAP or TRAPase), also called acid phosphatase 5, tartrate resistant (ACP5) or TRAP5b, is a glycosylated monomeric metalloprotein enzyme expressed in mammals.It has a molecular weight of approximately 35kDa, a basic isoelectric point (7.6–9.5), and optimal activity in acidic conditions. TRAP is synthesized as latent proenzyme and activated by proteolytic cleavage and reduction.It is differentiated from other mammalian acid phosphatases by its resistance to inhibition by tartrate and by its molecular weight.The mechanism of phosphate ester hydrolysis by TRAP is through a nucleophilic attack mechanism, whereby, catalysis occurs with the binding of a phosphate-substrate to the Fe2+ in the active site of TRAP.
Principle of the Assay:
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ACP5 / TRACP polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ACP5 / TRACP polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ACP5 / TRACP amount of sample captured in plate. Read the O. D. absorbance at 450nm in a microplate reader, and then the concentration of ACP5 / TRACP can be calculated.
Application:
For quantitative detection of ACP5 / TRACP in human serum, plasma, body fluids, tissue lysatesor cellculture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6months, or at -20℃for 12 months.
Tartrate-resistant acid phosphatase (TRAP or TRAPase), also called acid phosphatase 5, tartrate resistant (ACP5) or TRAP5b, is a glycosylated monomeric metalloprotein enzyme expressed in mammals.It has a molecular weight of approximately 35kDa, a basic isoelectric point (7.6–9.5), and optimal activity in acidic conditions. TRAP is synthesized as latent proenzyme and activated by proteolytic cleavage and reduction.It is differentiated from other mammalian acid phosphatases by its resistance to inhibition by tartrate and by its molecular weight.The mechanism of phosphate ester hydrolysis by TRAP is through a nucleophilic attack mechanism, whereby, catalysis occurs with the binding of a phosphate-substrate to the Fe2+ in the active site of TRAP.
Principle of the Assay:
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ACP5 / TRACP polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ACP5 / TRACP polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ACP5 / TRACP amount of sample captured in plate. Read the O. D. absorbance at 450nm in a microplate reader, and then the concentration of ACP5 / TRACP can be calculated.
Application:
For quantitative detection of ACP5 / TRACP in human serum, plasma, body fluids, tissue lysatesor cellculture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6months, or at -20℃for 12 months.
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