Human ALOX12 (Arachidonate-12-Lipoxygenase) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-12-Lipoxygenase(ALOX12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate-12-Lipoxygenase(ALOX12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-12-Lipoxygenase(ALOX12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arachidonate-12-Lipoxygenase(ALOX12) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase, Metabolic pathway, Neuro science, Developmental science

Alternative Names:
LOX12, 12-LOX, ALOX-12, 12-LO, 12LPG, LOG12, Lipoxygenase 12, 12S-lipoxygenase, Lipoxin synthase 12-LO, Platelet-type lipoxygenase 12

Sample Type:
tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 20 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only