Human ALOX15B (Arachidonate-15-Lipoxygenase, Type B) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-15-Lipoxygenase, Type B(ALOX15B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate-15-Lipoxygenase, Type B(ALOX15B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-15-Lipoxygenase, Type B(ALOX15B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arachidonate-15-Lipoxygenase, Type B(ALOX15B) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase, Metabolic pathway

Alternative Names:
LOX15B, 15-LOX-2, Arachidonate 15-lipoxygenase type II, Lipoxygenase 15, Type B, 15-lipoxygenase 2, Linoleate 13-lipoxygenase 15-LOb

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids.

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only