Human ANA (anti-nuclear Antibody) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANA(anti-nuclear Antibody). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ANA(anti-nuclear Antibody). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ANA(anti-nuclear Antibody), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ANA(anti-nuclear Antibody) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Biochemical research

Alternative Names:
Anti nuclear Antibody

Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 100 ng/mL

Assay Length: 3.5h

Assay Length: For Research Use Only