Human Anti-GH (Anti-Growth Hormone Antibody) ELISA Kit

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Anti-Growth Hormone Antibody(Anti-GH) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Anti-Growth Hormone Antibody(Anti-GH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Growth Hormone Antibody(Anti-GH) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Endocrinology, Neuro science, Hormone metabolism

Alternative Names:
GH1, GH-N, GHN, hGH-N, Somatotropin, Hygetropin, Jintropin, Kigtropin, Pituitary Growth Hormone, Growth Hormone, Normal

Sample Type:
Serum, plasma and other biological fluids.

Assay Type: Competitive Inhibition

Standard: 200 ng/mL

Assay Length: 2h, 30min

Assay Length: For Research Use Only