Human Anti-MBL (Anti-Mannose Binding Lectin Antibody) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with Mannose Binding Lectin(MBL) protein. Standards or samples are then added to the appropriate microtiter plate wells with Mannose Binding Lectin(MBL) protein. Then add a biotin-conjugated Mannose Binding Lectin(MBL) protein. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anti-Mannose Binding Lectin Antibody(Anti-MBL) , biotin-conjugated protein and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Mannose Binding Lectin Antibody(Anti-MBL) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction, Infection immunity, Immune molecule,

Alternative Names:
MBL2, COLEC1, HSMBPC, MBP1, MBP, Collectin-1, Mannose-binding protein C, Mannan Binding Protein, Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)

Sample Type:
Serum, plasma and other biological fluids

Assay Type: Sandwich

Standard: 400 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only