Human APC (Adenomatous polyposis coli) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to APC(Adenomatous polyposis coli). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to APC(Adenomatous polyposis coli). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APC(Adenomatous polyposis coli), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of APC(Adenomatous polyposis coli) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cell Biology, Neuroscience, Epigenetics and Nuclear Signaling

Alternative Names:
APC, DP2, DP3, FPC, GS, DP2.5, Protein APC, Min, adenomatosis polyposis coli, adenomatosis polyposis coli tumor suppressor, adenomatous polyposis coli, adenomatous polyposis coli protein, BTPS2, Deleted in polyposis 2.5

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids

Assay Type: Sandwich

Standard: 1000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only