Human ASCC3 (Activating Signal Cointegrator 1 Complex Subunit 3) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activating Signal Cointegrator 1 Complex Subunit 3(ASCC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activating Signal Cointegrator 1 Complex Subunit 3(ASCC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activating Signal Cointegrator 1 Complex Subunit 3(ASCC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activating Signal Cointegrator 1 Complex Subunit 3(ASCC3) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Tumor immunity

Alternative Names:
HELIC1, RNAH, ASC1p200, RNA Helicase Family, Helicase, ATP Binding 1, Trip4 complex subunit p200, ASC-1 complex subunit p200

Sample Type:
Tissue homogenates, cell lysates and other biological fluids.

Assay Type: Sandwich

Standard: 20 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only