Human b3GAT1 (Beta-1,3-Glucuronyltransferase 1) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-1,3-Glucuronyltransferase 1(b3GAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta-1,3-Glucuronyltransferase 1(b3GAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-1,3-Glucuronyltransferase 1(b3GAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-1,3-Glucuronyltransferase 1(b3GAT1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: CD & Adhesion molecule, Metabolic pathway, Neuro science, Hepatology

Alternative Names:
CD57, B3-GAT1, GLCATP, GlcAT-P, GlcUAT-P, HNK-1, LEU7, NK-1, Glucuronosyltransferase P, Galactosylgalactosylxylosylprotein 3-Beta-Glucuronosyltransferase 1

Sample Type:
Tissue homogenates, cell lysates and other biological fluids.

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only