Human BAK1 (Bcl2 Antagonist/Killer 1) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Antagonist/Killer 1(BAK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2 Antagonist/Killer 1(BAK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Antagonist/Killer 1(BAK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bcl2 Antagonist/Killer 1(BAK1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Apoptosis

Alternative Names:
BAK, BAK-LIKE, BCL2L7, CDN1, Bcl-2 Homologous Antagonist/Killer, Apoptosis regulator BAK, Bcl-2-like protein 7

Sample Type:
Tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 40 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only