Human BTLA (B- and T-lymphocyte attenuator) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B- and T-lymphocyte attenuator(BTLA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B- and T-lymphocyte attenuator(BTLA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B- and T-lymphocyte attenuator(BTLA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B- and T-lymphocyte attenuator(BTLA) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cardiac function, Cancer

Alternative Names:
B and T lymphocyte associated protein, B and T lymphocyte attenuator, B and T lymphocyte associated, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein, BTLA, BTLA_HUMAN, BTLA1, CD272, CD272 antigen, FLJ16065, MGC129743

Sample Type:
Serum, plasma, urine, tissue homogenates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 30 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only