Human C1r (Complement Component 1, R Subcomponent) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 1, R Subcomponent(C1r). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 1, R Subcomponent(C1r). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 1, R Subcomponent(C1r), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 1, R Subcomponent(C1r) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction, Infection immunity, Immune molecule,

Alternative Names:
C1-r, complement Component 1, Complement component 1 subcomponent r

Sample Type:
Serum, plasma and other biological fluids

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only