Human CA125/MUC16 ELISA Kit
€0.00
In stock
SKU
BEK1491
Catalog Number: BEK1491
Size(s): 96 tests/kit
Standard Range: 15.6 pg/ml - 1000 pg/ml
Sensitivity: <5 pg/ml
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Size(s): 96 tests/kit
Standard Range: 15.6 pg/ml - 1000 pg/ml
Sensitivity: <5 pg/ml
Request Information
Introduction:
Mucin-16 (MUC-16) also known as Ovarian cancer-related tumor marker CA125 is a protein that in humans is encoded by the MUC16 gene.MUC-16 is a member of the mucin family glycoproteins.MUC-16 has found application as a tumor marker or biomarker that may be elevated in the blood of some patients with specific types of cancers, most notably ovarian cancer, or other conditions that are benign.Mucin 16 is a membrane associated mucin that possesses a single transmembrane domain. A unique property of MUC16 is its large size. MUC16 is more than twice as long as MUC1 and MUC4 and contains about 22,000 amino acids, making it the largest membrane-associated mucin.
Principle of the Assay:
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CA125 / MUC16 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CA125 / MUC16 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CA125 / MUC16 amount of sample captured in plate. Read the O. D. absorbance at 450nm in a microplate reader, and then the concentration of CA125 / MUC16 can be calculated.
Application:
For quantitative detection of CA125 / MUC16 in human serum, plasma, body fluids, tissue lysatesor cellculture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6months, or at -20℃for 12 months.
Mucin-16 (MUC-16) also known as Ovarian cancer-related tumor marker CA125 is a protein that in humans is encoded by the MUC16 gene.MUC-16 is a member of the mucin family glycoproteins.MUC-16 has found application as a tumor marker or biomarker that may be elevated in the blood of some patients with specific types of cancers, most notably ovarian cancer, or other conditions that are benign.Mucin 16 is a membrane associated mucin that possesses a single transmembrane domain. A unique property of MUC16 is its large size. MUC16 is more than twice as long as MUC1 and MUC4 and contains about 22,000 amino acids, making it the largest membrane-associated mucin.
Principle of the Assay:
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CA125 / MUC16 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CA125 / MUC16 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CA125 / MUC16 amount of sample captured in plate. Read the O. D. absorbance at 450nm in a microplate reader, and then the concentration of CA125 / MUC16 can be calculated.
Application:
For quantitative detection of CA125 / MUC16 in human serum, plasma, body fluids, tissue lysatesor cellculture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6months, or at -20℃for 12 months.
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