Human CCL14 (Chemokine C-C-Motif Ligand 14) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Ligand 14(CCL14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Ligand 14(CCL14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-C-Motif Ligand 14(CCL14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-C-Motif Ligand 14(CCL14) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cytokine, Tumor immunity, Infection immunity, Immune molecule

Alternative Names:
SCYA14, HCC-1, HCC-3, NCC-2, SCYL2, CKb1, MCIF, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 14

Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 1000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only