Human CCR4 (Chemokine C-C-Motif Receptor 4) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Receptor 4(CCR4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Receptor 4(CCR4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-C-Motif Receptor 4(CCR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-C-Motif Receptor 4(CCR4) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction, CD & Adhesion molecule, Infection immunity, Rheumatology,

Alternative Names:
CD194, CC-CKR-4, CKR4, CMKBR4, ChemR13, K5-5

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only