Human CD200R1 (CD200 Receptor 1) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD200 Receptor 1(CD200R1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CD200 Receptor 1(CD200R1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD200 Receptor 1(CD200R1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CD200 Receptor 1(CD200R1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: CD & Adhesion molecule

Alternative Names:
OX2R, HCRTR2, CD200R, MOX2R, MOX2 Receptor, Cell surface glycoprotein CD200 receptor 1, CD200 cell surface glycoprotein receptor, Cell surface glycoprotein OX2 receptor 1

Sample Type:
Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 1000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only