Human CD74 (HLA class II histocompatibility antigen gamma chain) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD74(HLA class II histocompatibility antigen gamma chain). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CD74(HLA class II histocompatibility antigen gamma chain). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD74(HLA class II histocompatibility antigen gamma chain), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CD74(HLA class II histocompatibility antigen gamma chain) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Immunology

Alternative Names:
CD74, HLA class II histocompatibility antigen gamma chain, CLIP, DHLAG, HLADG, Ii, II, MHC HLA-DR gamma chain, p33, Ia-gamma, INVG34, CD74 antigen, HLA-DR antigens-associated invariant chain, HLA-DR-gamma, Ia antigen-associated invariant chain, Ia-associated invariant chain

Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 40 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only