Human CD97 (CD97 antigen) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD97(CD97 antigen). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CD97(CD97 antigen). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD97(CD97 antigen), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CD97(CD97 antigen) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cancer

Alternative Names:
CD97, CD97 antigen, CD97 molecule, Leukocyte antigen CD97, TM7LN1

Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only