Human CK-18 (Cytokeratin 18) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CK-18(Cytokeratin 18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CK-18(Cytokeratin 18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CK-18(Cytokeratin 18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CK-18(Cytokeratin 18) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cancer of the liver, Viral hepatitis

Alternative Names:
Cell proliferation inducing gene 46 protein , Cell proliferation inducing protein 46 , Cell proliferation-inducing gene 46 protein , CK 18 , CK-18 , CK18 , CYK 18 , CYK18 , Cytokeratin 18 , Cytokeratin endo B , Cytokeratin-18 , K 18 , K18 , K1C18_HUMAN , KA18 , Keratin 18 , Keratin 18, type I , Keratin D , keratin, type I cytoskeletal 18 , Keratin-18 , Krt18

Sample Type:
Tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only