Human CKMT1A (Creatine Kinase, Mitochondrial 1A) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Creatine Kinase, Mitochondrial 1A(CKMT1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Creatine Kinase, Mitochondrial 1A(CKMT1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Creatine Kinase, Mitochondrial 1A(CKMT1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Creatine Kinase, Mitochondrial 1A(CKMT1A) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cardiovascular biology

Alternative Names:
CKMT, CKMT1-A, Mia-CK, U-MtCK, Creatine kinase U-type, mitochondrial, Ubiquitous mitochondrial creatine kinase, Acidic-type mitochondrial creatine kinase

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids.

Assay Type: Sandwich

Standard: 100 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only