Human Clusterin ELISA Kit
€0.00
In stock
SKU
BEK1363
Catalog Number: BEK1363
Size(s): 96 tests/kit
Standard Range: 780 pg/ml - 50,000 pg/ml
Sensitivity: <260 pg/ml
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Size(s): 96 tests/kit
Standard Range: 780 pg/ml - 50,000 pg/ml
Sensitivity: <260 pg/ml
Request Information
Introduction:
Clusterin is a protein that in humans is encoded by the CLU gene on chromosome 8.[5] CLU is an extracellular molecular chaperone which binds to misfolded proteins in body fluids to neutralise their toxicity and mediate their cellular uptake by receptor-mediated endocytosis. Once internalised by cells, complexes between CLU and misfolded proteins are trafficked to lysosomes where they are degraded.CLU is involved in many diseases including neurodegenerative diseases, cancers, inflammatory diseases, and aging.The CLU gene contains nine exons and a variety of mRNA isoforms can be detected, although most of these are only ever expressed at very low levels (< 0.3% of the total). The full-length mRNA encoding the secreted isoform is by far the dominant species transcribed.
Principle of the Assay:
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-clusterin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-clusterin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the clusterin amount of sample captured in plate. Read the O. D. absorbance at 450nm in a microplate reader, and then the concentration of clusterin can be calculated.
Application:
For quantitative detection of cluster in in human serum, plasma, body fluids, tissue lysatesor cellculture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6months, or at -20℃for 12 months.
Clusterin is a protein that in humans is encoded by the CLU gene on chromosome 8.[5] CLU is an extracellular molecular chaperone which binds to misfolded proteins in body fluids to neutralise their toxicity and mediate their cellular uptake by receptor-mediated endocytosis. Once internalised by cells, complexes between CLU and misfolded proteins are trafficked to lysosomes where they are degraded.CLU is involved in many diseases including neurodegenerative diseases, cancers, inflammatory diseases, and aging.The CLU gene contains nine exons and a variety of mRNA isoforms can be detected, although most of these are only ever expressed at very low levels (< 0.3% of the total). The full-length mRNA encoding the secreted isoform is by far the dominant species transcribed.
Principle of the Assay:
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-clusterin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-clusterin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the clusterin amount of sample captured in plate. Read the O. D. absorbance at 450nm in a microplate reader, and then the concentration of clusterin can be calculated.
Application:
For quantitative detection of cluster in in human serum, plasma, body fluids, tissue lysatesor cellculture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6months, or at -20℃for 12 months.
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