Human COL1A1 (Pro-Collagen I alpha 1) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to COL1A1(Pro-Collagen I alpha 1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to COL1A1(Pro-Collagen I alpha 1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL1A1(Pro-Collagen I alpha 1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of COL1A1(Pro-Collagen I alpha 1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal Transduction

Alternative Names:
Alpha 1 type I collagen, Alpha 2 type I collagen, alpha 2 type I procollagen, alpha 2(I) procollagen, alpha 2(I)-collagen, Alpha-1 type I collagen, alpha1(I) procollagen

Sample Type:
serum, plasma and other biological fluids

Assay Type: Sandwich

Standard: 2500 pg/mL

Assay Length: 3.5h

Assay Length: For Research Use Only