Human COL5 (Collagen Type V) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to COL5(Collagen Type V). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to COL5(Collagen Type V). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL5(Collagen Type V), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of COL5(Collagen Type V) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Metabolic pathway, Developmental science, Nutrition metabolism, Bone metabolism

Alternative Names:
Collagen Type V

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids

Assay Type: Sandwich

Standard: 200 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only